eISSN: 1643-3750


Application of studies of nucleolar organizer regions (NORs) to clinical and morphological diagnosis, particularly in lung cancer

Daniel Sabat, Jerzy K. Nożyński, Zbigniew Szczurek, Andrzej Gabriel

Med Sci Monit 1996; 2(6): CR720-726

ID: 500125

Published: 1996-11-01

Expression marking of nucleolar organising regions (NORs) is a modern quantitative diagnostic method applied more widely in evaluation of proliferating activity of tumour cells. A particular interest has been observed since 1986, i.e., since a report by Ploton on a simple colloid method of one-stage protein silverstaining. This allowed a quantitative evaluation of the regions on light microscopy. The present study describes application of marking of argyrophil granules (Ag-NORs) in different tumours and precancerous stages mainly to differentiate borderline lesions. Particular emphasis is paid to the use of the granulation marking in diagnosing small and non-small cell lung carcinoma. The study presents a number of controversies on dependence of Ag-NOR expression on the histological type, clinical stage and tumour mass doubling time as well as views on little usefulness of Ag-NORs in differentiating particular types of carcinomas. Alternating study methods of granules in the cell nucleus from tissue and cytological material, the application of computer analysis and Ag-NOR method assisted by immunohistochemical techniques are also discussed.

Keywords: nucleolar organiser regions, lung cancer